Nm To Ng Ul Calculator. Email: sequence@functionalbio. Input the molecular weight and

Email: sequence@functionalbio. Input the molecular weight and nanogram concentration for accurate results in nanomolarity. Library Size (bp) Description (Optional) Library Concentration (ng/µl) Library Concentration (nM) Library 1 Convert Concentration Copy Calculations Converting concentrations from ng/μl to nM is a common task in molecular biology labs. This is commonly used in molecular biology and biochemistry to quantify the concentration of DNA, RNA, or protein in a sample. This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for nucleic acids, and vice versa. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. Calculator: Convert Nanomolar (nM) To achieve the desired result as quickly as possible, it is best to enter the value to be converted as text, for example '36 nM to M' or simply '32 nM '. Dec 5, 2024 · Enter the concentration, mass of substance, and volume into the calculator to determine the missing variable. Mar 17, 2011 · So I have a DNA sample that's 330 base pairs in size with a concentration of 51 ng/ul. You can view more details on each measurement unit: ng/ul or nanogram/milliliter The SI derived unit for density is the kilogram/cubic meter. Oct 3, 2024 · The Ng/Ml to Nm Calculator is a useful tool for converting concentrations from nanograms per milliliter (ng/ml) to nanomolar (nM), which is essential for many biochemical and pharmacological applications. molar quantity (mol) --> Molar concentration (M) Progesterone ng/mL nmol/L Conversion in Batch ng/mL: nmol/L: Decimals 1 Decimals 2 Decimals 3 Decimals 4 Decimals 5 Decimals 6 Decimals 7 Decimals 8 Decimals 9 Decimals 10 Decimals 11 Decimals 12 Decimals 13 Decimals No Decimal Convert DNA concentration (ng/µL, µg/mL) to molarity (mol/L, µM, nM) and molecules/µL. Converting from micrograms (µg) to nanomoles (nmol) to picomoles (pmol) to nanomolar to milimolar can be confusing. VOTE David Aucoin Follow A simple formula is as follow: [ (concentration in ng/ul0]/ (660g/mol x average size of the of the dsDNA-determined by Bioanalyzer or Tapestation )] x 1000000 = Conc. , micromolar (µM, 10 -6 M) or nanomolar (nM, 10 -9 M). Sep 18, 2025 · What is a Ng/Ml To Molarity? Ng/ml to molarity is a conversion process used in chemistry to change the concentration of a solution from nanograms per milliliter (ng/ml) to molarity (M). The goal of this short tutorial is to show you a way to easily inter-convert these values while standing in front of your mass spectrometer Oct 3, 2024 · Converting concentrations from picograms per microliter (pg/µL) to nanograms per microliter (ng/µL) is essential in various scientific fields, including biochemistry and pharmacology. Ideal for scientific labs and industrial applications. Whether you start with an OD₂₆₀ reading, a mass concentration, or a molar value, this tool quickly calculates the equivalents. Oct 1, 2025 · All Chemistry Calculators Ng/Ul Dilution Calculator Ng/Dl To Nmol/L Calculator Nmol/L To Ng/Dl Ng/µL To Molar Formula The following formula is used to calculate the molarity from concentration and molecular weight. 8 ng/uL sample concentration by this value to get the molarity of your solution, in M. High performance NGS solutions with flexible, efficient workflows for your research insights. This is often used in scientific research, particularly in the fields of chemistry and biology, to convert between these two units of concentration. Molarity is a measure of the number of moles of a solute per liter of solution, while ng/ml measures the mass of the solute per volume of solution. I've been told I need 10ul of a 10nM pool for each run but I'm not sure how to convert my individual concentrations (currently in ng/ul) into nano Moles? Oct 3, 2024 · Can I use this calculator for other concentrations? Yes, this calculator can be used for any dilution where concentrations are expressed in ng/µl, though the method applies to any unit of concentration as long as units are consistent. » ug/uL [microgram/uL] » ng/uL [nanogram/microliter] » ng/dL [nanogram/deciliter] » g/m3 [gram/cubic meter] » lb/ft3 [pound/cubic foot] » g/dL [gram/deciliter] » ng/L [nanogram/liter] » mg/tsp [milligram/teaspoon] » lb/gal (US) [pound/gallon (US)] » lbs/in3 [pound/cubic inch] » pg/uL [picogram/microliter] » oz/in3 [ounce/cubic inch]. Choose the organism you DNA originates from, or choose "Custom DNA fragment". Consider the xGen™ Normalase™ Module and xGen Normalase indexing primers for enzymatic normalization of up to 1,536 libraries. In Calculator: Convert Microgram per milliliter to Nanogram per milliliter (µg/ml to ng/ml) To achieve the desired result as quickly as possible, it is best to enter the value to be converted as text, for example '95 ug/ml to ng/ml ' or '67 ug/ml into ng/ml ' or simply '39 ug/ml '. Westlab's Nucleic Acid Concentration & Molar Converter provides a seamless way to switch between all common units used for quantifying DNA and RNA. , Units/mg). Calculate DNA concentration in your samples with the DNA Concentration Calculator for precise lab work results. NM to NG Calculator Use this calculator to convert nanomoles (nm) to nanograms (ng) based on a specified molecular weight. 1 kilogram/cubic meter is equal to 1000 ng/ul, or 1000000 nanogram This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μl If you know that the weight of your DNA (molar mass per bp) is different from the average weight of DNA basepair, change the value. How to calculate concentration in nM from ng/ul qubit concentration and size base pairs after bioanalyser results in ChiP sequencing? ChIP sequencing please put the information in the box Powered by Ubuntu and PHP5 Terms and Disclaimer Concentration solution unit conversion between microgram/uL and nanogram/microliter, nanogram/microliter to microgram/uL conversion in batch, ug/uL ng/uL conversion chart Oct 1, 2025 · Enter the amount in nmol and the molecular weight to convert to ng using this calculator. Dilution Calculator This tool will calculate the amount of stock solution needed to achieve a desired concentration in a given volume. These methods typically measure dsDNA concentration in ng/µl. You can view more details on each measurement unit: microgram/ml or nanogram/microlitre The SI derived unit for density is the kilogram/cubic meter. 1 kilogram/cubic meter is equal to 1000 microgram/ml, or This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μL. Sep 9, 2025 · Lab Math Solved: uM to ng/ul Conversion Calculator & Guide Published on 09 September 2025 in Guide 34 minutes on read 1- How to convert ng/ul concentration to pmol/ul? 2- How to calculate the amount of insert DNA from the above? This tool converts values from nanometers to nanograms quickly and accurately. CONVERSION TABLE Mar 31, 2021 · These methods typically measure dsDNA concentration in ng/µl. Library Size (bp) Description (Optional) Library Concentration (ng/µl) Library Concentration (nM) Library 1 Convert Concentration Copy Calculations 1. Enter the concentration of your stock solution, and the concentration and volume of the desired working solution. For further assistance, it directs users to More information from the unit converter How many microgram/ml in 1 nanogram/microlitre? The answer is 1. Use our DNA concentration calculator to convert A260 readings to ng/µL for dsDNA, ssDNA, or RNA, and convert between mass and molar concentration (nM) by fragment length. Whether you’re working in pharmacology, biochemistry, or environmental science, converting between units like nanograms, micrograms, milligrams, and grams per milliliter is essential for accuracy. ng/ml to Microgram/uL (ug/uL), Concentration Solution unit conversion You can assume an average molar mass of 660 g per bp. in nM I hope this helps VOTE Ben Warmus Follow Concentration solution unit conversion between nanogram/microliter and picogram/microliter, picogram/microliter to nanogram/microliter conversion in batch, ng/uL pg/uL conversion chart This converter is used to convert the molar quantity into the molar concentration, and vice versa. To convert from ng/μl to nM for cluster generation, follow the instructions below. The Ng/μl Dilution Calculator is a vital tool in biochemical laboratories, facilitating the accurate preparation of dilutions by calculating the final Sep 10, 2025 · Ng/ml to Nm is a conversion from nanograms per milliliter to nanomolar. This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μL. Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. DNA μg & pmol Converter This tool converts micrograms of DNA and picomoles of DNA according to the following formula where N is the length of the DNA: First enter the length of the DNA fragment in bp, then enter the amountof DNA either in ug (Top) or in pmol (Bottom) and click on the 'Calculate' button to make the unit conversion: From that you can convert to ng/uL for a 1 M solution. How to convert ng/µl to nanomolar (nM) when calculating ssDNA aptamers concentration? I used a nanodrop spectrophotometer to calculate ssDNA concentration (ng/µl). Multiply the nM indicator above for your average size (from the Bioanalyzer) by the Qubit concentration reading (ng/ul) to generate the Illumina converted concentration value for each sample. In order to convert from a mass/volume (concentration) to a molarity, you need to use the g/mol of the More information from the unit converter How many ng/ul in 1 nanogram/milliliter? The answer is 0. Input an initial concentration value/unit, initial or final volume, select unit you want to convert to, then press “CALCULATE”. m is the mass (i. It emphasizes the use of dsDNA-specific fluorescent dye methods for accurate quantification and suggests determining the average library size using an Agilent Technologies 2100 Bioanalyzer. 7), not only DNA is contaminated with proteins but the DNA concentration is A LIE and you should better use a method that specifically reads DNA, i. Below are calculations to convert between these units. What is the DNA Concentration Calculator? Definition: This calculator computes the concentration of a nucleic acid sample (DNA or RNA) based on its absorbance at 260 nm (A 260), using the Beer-Lambert Law. Jan 13, 2021 · These methods typically measure dsDNA concentration in ng/µl. Perfect for lab and clinical use! This calculator will determine the volume of stock solution required to make a working solution of a specific concentration and volume. Apr 30, 2005 · C is the desired concentration of the final solution with the concentration unit expressed in units of activity per volume of solution (e. below 1. Com mini tutorial" . This calculator can also evaluate any of the variables given the others are known. Nov 25, 2025 · This ng/mL calculator is designed to help students, researchers, and lab professionals convert concentrations of substances with ease. Nucleic Acid Concentration & Purity Converter Convert between OD₂₆₀, mass (ng/µL, µg/mL, µg/µL), molarity (µM, pmol/µL, nM), and copy number for dsDNA, ssDNA, or RNA. In most cases, the word 'to' (or '=' / '->') can be omitted between the names of the two units, for example '37 ng Solution Dilution Calculator This calculator is a tool for an accurate solution dilution. DNA copy number calculator determines the number of copies of single or double-stranded DNA and RNA in a solution and per PCR cycle. In most cases, the word 'to' (or '=' / '->') can be omitted between the names of the two units, for example '82 ug DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. Mar 15, 2024 · The DNA Dilution Calculator helps scientists and researchers accurately dilute their DNA samples to a desired concentration. We assume you are converting between microgram/milliliter and nanogram/microliter. To convert from ng/µl to nM for cluster generation, follow the instructions below. Drug Concentration Calculator* This calculator converts concentration between ng/mL and nM: n M = n g m L ∗ 1000 M W 660 is approximately the average mass of a single base pair. This tool was developed to allow quick conversion from nmol or pmol to µg, as well as µg to nmol or pmol. But, many techniques (including DNA sequencing) require a known number of molecules (moles or nanomoles) to be added. You can divide your 1284. Perform precise NG UL dilution calculations effortlessly with this accurate calculator. Whether you are double-checking your calculations or need a reliable conversion tool, this calculator will do the trick! The Ng/mL to nM Calculator allows you to quickly convert a concentration given in ng/mL to nM by inputting two key pieces of information: Concentration in ng/mL (nanograms per milliliter): This is the concentration of the substance in the solution, typically provided on product labels or calculated from experimental data. Unit of Measure for Library nM ng/µl Pooled Library Concentration (nM) Total Pooled Library Volume (µl) Description (optional) Do I use beer-lamberts law or calculate concentration from ng/ul concentration using molecular weight of the oligos? Both calculations give me VERY different results for concentration. Then you can convert from M to uM. Normally, our concentrations are given in units of milligrams per milliliter (mg/ml), but often data sheet quality assurance data are expressed in molarity (moles per liter, M) or fractions of that — e. Or view the worksheet. Use the following formula to convert from ng/μl to nM: Jun 2, 2018 · I want to treat my cell with 10, 25 and 50 ng/ml concentration (500 ul each) but I cant figure how to calculate the amount of reagent that need to be used. e. , weight) of solute that must be dissolved in volume V of solution to make the desired Dec 27, 2024 · Convert ng/mL to nM with the Ng/mL to nM Calculator. Jan 19, 2016 · Impress Your Friends, Win Adulations by Learning to "Calculate on Your Feet" (even with your shoes on) "an IonSource. 1. 001. Determine the average size of the library by running it on an Agilent Technologies 2100 Bioanalyzer. 3. fluorimetry. Convert between nanogram/milliliter and nanogram/microliter with this tool, including batch conversion and a handy ng/ml to ng/μL chart. Optionally calculates A260/A280 ratio. Some standard Illumina libraries, such as Nextera, require the use of dsDNA-specific fluorescent dye methods for accurate quantification. But when A260/A280 is bad (i. As I know, we calculate the mass concentration of nucleic acids by spectrometre which gives it as ng/ul. If you choose a custom please put the information in the box Powered by Ubuntu and PHP5 Terms and Disclaimer Convert Nanogram/microliter to Other Concentration solution Unit: » 1 ng/uL = 1 mg/L [milligram/liter] » ng/uL ↔ mg/mL [milligram/mL] » ng/uL ↔ mg/dL [milligram/deciliter] » ng/uL ↔ g/L [gram/liter] » ng/uL ↔ g/cm3 » 1 ng/uL = 1 ug/mL [microgram/mL] This page provides unit conversion tools for nanogram per microliter (ng/uL), a concentration measurement. Can anyone walk me through how to make a 10 nm dilution? home>> tools>>Unit Conversion (uM to ng/ul) uM to ng/ul Calculator: Convert Nanogram per milliliter to Microgram per milliliter (ng/ml to µg/ml) To achieve the desired result as quickly as possible, it is best to enter the value to be converted as text, for example '77 ng/ml to ug/ml ' or '67 ng/ml into ug/ml ' or simply '57 ng/ml '. Sep 11, 2025 · Enter the initial concentration in ng/ul, initial volume in ul, and final volume in ul into the calculator to determine the final concentration after dilution. The need arises because quantification methods for DNA typically measure the mass per volume of nucleotides in a sample. A is the activity of the solid material with the activity unit expressed in units of activity per mass of material (e. Otherwise just use the default value. So TL;DR: in a clean sample, A260*50 = DNA concentration in ng/ul. Easy DNA molarity calculator with formula, chart, and export options. A calculator for this task is available at http://ww Converting concentrations from ng/μl to nM is a common task in molecular biology labs. com Linkedin Services Full Plasmid Sequencing Sanger Sequencing Plasmid Purification Picking and Growing Exo/SAP Cleanups PCR Primer Walking Ready to Inject Samples Fragment Analysis Resources Sample Submission Guidelines Dilution & Solution Calculators FAQs Links News & Promotions Sitemap Access our free medical lab calculator formulas for converting relevant units. Unlock accurate DNA measurements, molarity conversions, and dilution protocols with this all-in-one calculator—whether you’re preparing samples for PCR, sequencing, or cloning. These methods typically measure dsDNA concentration in ng/μl. Library Concentration Conversion Calculator Consider the xGen™ Normalase™ Module and xGen Normalase indexing primers for enzymatic normalization of up to 1,536 libraries. Enter the molar mass for conversions between molar concentration and %. Use the full power of this unit converter to convert nM With this calculator, it is possible to enter the value to be converted together with the original measurement unit; for example, '195 Nanomolar'. Solution density is assumed to be “1”. At this stage if you know the concentration in ng/uL and the MW, you don't need to use the EC for anything to determine the Sep 15, 2010 · Hi all, I've just finished preparing 72 samples for sequencing, these have prepared using index primers and so I want to pool them together before running on the sequencer. 2. Concentration molar unit conversion between microMolar and nanomole/microliter, nanomole/microliter to microMolar conversion in batch, uM nmol/uL conversion chart Jun 2, 2018 · I want to treat my cell with 10, 25 and 50 ng/ml concentration (500 ul each) but I cant figure how to calculate the amount of reagent that need to be used. g. Converting ng/μl to nM When Calculating dsDNA Library Concentration Standard Illumina libraries, libraries having undergone PCR amplification, require the use of dsDNA-specific fluorescent dye Dec 1, 2022 · How to convert ng/ul to nM (ssRNA)? I have a solution of ssRNA molecules and want to know how to convert the ng/ul concentration to nM (nano moles). Convert Nanogram/microliter to Other Concentration solution Unit: » 1 ng/uL = 1 mg/L [milligram/liter] » ng/uL ↔ mg/mL [milligram/mL] » ng/uL ↔ mg/dL [milligram/deciliter] » ng/uL ↔ g/L [gram/liter] » ng/uL ↔ g/cm3 » 1 ng/uL = 1 ug/mL [microgram/mL] Apr 30, 2023 · The reaction number can make it easier to calculate the quantity of a given product for your experiment, if your experiment is performed according to the supplier’s directions. The dilution process involves reducing the concentration of the substance in the solution, often to make it Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. We assume you are converting between nanogram/microliter and nanogram/millilitre. Illumina Miseq concentration calculation: Multiply the nM indicator above for your average size (from the Bioanalyzer) by the Qubit concentration reading (ng/ul) to generate the Illumina converted concentration value for each sample. ng/ml to ng/uL, Concentration Solution unit conversion The document provides instructions for converting dsDNA library concentration from ng/µl to nM, which is necessary for cluster generation in Illumina sequencing. What is a Ng/Ul Dilution? A Ng/Ul dilution refers to the concentration of a particular substance in a solution, measured in nanograms (Ng) per microliter (Ul). Use the following formula to convert from ng/μl to nM: ( Concentration in ng/μl ) Example calculation for converting ng/ul to nM and performing a dilution to get a desired concentration. Labs that run half reactions or that extensively customize their experiments may have difficulty calculating how much oligo is needed for a given reaction. , Units/mL).

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